Unreliable testing -
Residue analysis called into question

The Working Party on Pesticide Residues has found large variations in residue test results. For the first time, its annual report shows which retailers are selling foods that exceed statutory residue limits.

Excessive residues
In September each year the Working Party on Pesticide Residues (WPPR) (soon to be renamed the Pesticides Residues Committee) publishes its annual report. This year's results showed 25% of food analysed contained pesticide residues at levels below the maximum residue levels (MRLs) and 1.4% of samples exceeded the MRL (see table 1). These figures should be treated carefully, as the percentage of food samples that contain pesticides will depend on what foods are sampled and what pesticides are sought.

Table 1. Recent sampling results
  1995 1996 1997 1998
No. of samples 3230 2263 1878 2200
Containing
residues (%)
31 34 29 25
Exceeding MRLs (%) 1 1 1 1.4

A large proportion of the excess residues were found in yams, sampled for the first time. Twelve out of 16 samples contained car-bendazim technically in excess of MRLs. This is because no MRLs have been applied for by the importers, so any residue found is treated as exceeding the MRL.
   
More serious exceedances of MRLs occurred in pears with high levels in imports from Belgium and Holland, and evidence of illegal use of pesticides by UK growers (see end).
   
The illegal use of certain fungicides on lettuce has been a problem for the past six years. This year two out of 99 samples contained chlorothalonil, and six out of 11 iprodione, neither of which are approved for use on winter lettuce.

Unpredictable results
Chlorothalonil is a fungicide that is difficult to analyse accurately. This is because it is unstable and degrades during analysis. It now seems the problem is wider than just chlorothalonil. Tests run in 1999 on four representative commodities-apples, lettuce, oranges and tomatoes-and spiked with over 100 pesticide residues, showed that residue 'loss' occurred during processing (see table 2). The 'loss' of residues means that results may understate the amount of pesticide actually in the sample.
   
These results are highly significant. It is not clear why different laboratories are getting different results, why different pesticides behave differently, nor why the losses occur in the first place. One idea is that either the temperature at which the sample is analysed (from frozen, or at room temperature), or the fineness with which the sample is mashed for analysis may be important. Clearly, the interpretation of residue analysis needs to be taken with a pinch of salt, and there may be more pesticides in food than we think.

Table 2. Loss of pesticide residues from spiked samples during analytical processing
  No. of pesticides sought No. of pesticides with loss > 20%
    Lab 1 Lab 2 Lab 3
Apple 105 16 33 69
Lettuce 104 81 86 13
Orange 106 12 17 8
Tomato 106 14 5 3

Naming the guilty parties
In accordance with the policy of 'openness' espoused by government Ministers, this report names the retail outlets where samples containing residues in excess of MRLs are bought. This policy requires the Pesticides Safety Directorate (PSD) to notify the retail outlet, which has the opportunity to reply. The gist of the replies are then published. For example, five samples of imported spinach had residues in excess of the MRLs. One sample from Sainsbury, and one from ASDA contained excess levels of the insecticide permethrin; and one sample each from Safeway, Sainsbury and Tesco, had levels of the fungicide iprodione above the MRL.

Choosing the samples
Until recently, samples were bought at the front of the shop, to ensure that the food chosen was the same as the consumer would actually buy. If offending brands are now going to be named, then sampling should now be 'at the back of the shop' where lots-particularly of fruit and vegetables-from different suppliers come in, in labelled boxes, and are mixed: this is the point at which traceability can be demonstrated.

What's next?
WPPR tries to target its resources at a combination of regular dietary staples, rolling programmes of food samples, and new areas that have not previously been looked at. New areas in the future will include fast food burgers, bottled water and pasta, and more emphasis will be put on monitoring fruit and vegetables commonly given to children. And how much reliance can we put on the results?

Annual Report of the WPPR 1998, Ministry of Agriculture Fisheries and Food/Health and Safety Executive, MAFF Publications, London, 1999, 100pp. WPPR results are also available on: www.maff.gov.uk/aboutmaf/agency/psd/wppr97/wppr1.htm

Pesticides in your pears
High levels of the pesticide chlormequat (a growth regulator, which helps to swell pears, and ensure that most of the crop on the tree ripens ready to drop at the same time) have been found in pears imported from Belgium and Holland, indicating large scale overuse.
   
Chlormequat has also been found in UK pears where it is not approved for use. Four out of five samples of these pears contained chlormequat at levels well below MRLs. Another sample contained the pesticide tolylfluanid, which is also not approved for use on pears. These findings indicate illegal use of both chlormequat and tolylfluanid. There has been concern since 1995 that chlormequat was being used illegally by UK growers.
   
Six out of 36 samples of imported pears contained chlormequat above the MRL of 3 mg/kg. It is heavily used in Belgium and Holland. The Pesticides Safety Directorate (PSD) notes that: "assurances have been received that the authorities have begun a more rigorous screening programme with the aim of ensuring that future imports to the UK will fall within statutory MRL limits."
   
PSD states that at the highest level of chlormequat residue found (11 mg/kg - or nearly four times the MRL) there should be no health risk to consumers, even in the 'worst case assumption' (a toddler consuming large quantities of pears); and that 'adverse health effects are most unlikely although the safety margins could be eroded.'
   
Regulators are now concerned not just about long-term low doses, but also by short-term higher doses. Research shows that the Acute Reference Dose (acuteRfD)-the safe dose at one meal or on one day for the most vulnerable population group (probably children) based on toxicological data-is about 0.05 mg/kg/body weight. So for an 'average' adult weighing 70 kg, the lifetime safe dose would be 3.5 mg. Anyone eating more than one pear a day with high residues may be vulnerable. It is difficult to assess what the chances of this may be: but clearly pears are not as safe as we thought they were. This is another instance of too many chemicals in fruit.

[This article first appeared in Pesticides News No. 45, September 1999, page 6]